A novel Arabidopsis phyllosphere resident Protomyces species and a re-examination of genus Protomyces based on genome sequence data

Abstract Protomyces is an understudied genus of yeast-like fungi currently defined as phytopathogens only Umbelliferae and Compositae . Species relationships boundaries remain controversial molecular data are lacking. Of the 82 named Protomyces, we found few recent studies six available cultures. We previously isolated strains from wild Arabidopsis thaliana, a member Brassicaceae , family distant accepted hosts. sequenced genomes all species, P. arabidopsidicola sp. nov. strain C29, Phylogenomics suggests this new species occupied unique position in genus. Genomic, morphological, physiological characteristics distinguished other Nuclear gene phylogenetic marker analysis actin1 DNA sequences could be used with nuclear ribosomal internal transcribed spacer for rapid identification species. Previous demonstrated persist on phyllosphere were discovered at multiple sites different countries. conclude that C29 represents novel propose name sp Consequently, not strictly associated recognized host plants.